Dr. J. K. Haynes
David Packard Professor and Dean of
Science and Mathematics
·
Education
·
Courses Taught
·
Research Interests
·
Publications
Education:
B.S.:Biology,Morehouse
College, 1964
Ph.D.: Developmental
Biology, Brown University,
1970
Ph.D Thesis: Analysis of
Tryptophan Oxygenase in the Frog, Rana pipiens
Courses Taught:
Cell Biology (BIO 251) 03 – 04 (Syllabus)
Biological Research I and II (BIO
381-382)
Senior Seminar in Biology (BIO
425)
Research Interests:
Cell
Biology
Cell Membranes
Biochemical characterization of sickle cell
membranes
Regulation of cell volume
The symptoms of sickle cell anemia are due
primarily to vaso-occusions, which result from red blood cell membrane
damage. We have detected several alterations in the protein composition of
sickle cell membranes, including the appearance of a unique high molecular
weight protein. Continuing investigations aim at further characterization of
these protein alterations and determining whether they play a role in
vaso-occlusions.A wide variety of cells have been shown to maintain their
volume in anisosmotic medium by regulating the activity of specific membrane
transport systems. In marine fishes and in vertebrates, membrane transport of
organic osmolytes, particularly amino acids, is principally responsible for
the regulation of cell volume. In elasmobranchs, the key organic osmolytes
are the (-amino acids, taurine and (-alanine. We have shown that skate
erythrocytes regain their volume after swelling in hypotonic medium by
releasing taurine through a gated-membrane channel, whose size appears to be
about 6 Ao in diameter. We have also studied osmoregulation in shark rectal
gland cell cultures. Inhibitor studies suggest that a different protein is
involved in taurine transport in these cells and that a tyrosine
phosphoprotein is involved. The goal of future work is to further
characterize the regulation and mechanism of action of osmoregulatory
transport proteins.
Selected Publications:
Gunn, R.B. and Haynes, J.K. (2002). KC1 cotransport
is increased in nearly all sickle red cells not just the reticulocytes or a
small subfraction. Presented at the Annual Meeting of the Society for
General Physiology, Woods Hole, September.
Haynes, J.K. (2002). Linking Departmental and
Institutional Mission, in Building Robust Environments in Undergraduate
Science Technology, Engineering and Mathematics, (Jeanne Narum, ed).,
Jossey-Bass, San
Francisco.
Haynes, J.K., Sanderlin, D., Omulepu, O., Lehrich, R.,
and Forrest, Jr., J.N. 1996. Volume Activated Taurine Efflux from Squalus
Acanthias Rectal Gland Cells. Mount
Desert Island Biological
Laboratory Bulletin 35:79-82.
Haynes, J.K. and Goldstein, L. 1993. Volume Regulatory
Amino Acid Transport in Erythrocytes of the Little Skate Raja erinacea. Am.
J. Physiol. 34:R173-R179.
How
to Earn Honors in Biology
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Department of
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Morehouse
College
830
Westview Dr. SW
Atlanta,
GA 30314
Office: 104 Nabrit-Mapp-McBay Science Building
(404) 215-2610
FAX (404) 522-9564
jhaynes@morehouse.edu
Last Updated: 6-28-03
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